Kinetic Studies of Adenine Phosphoribosyltransferase
نویسندگان
چکیده
منابع مشابه
Kinetic studies of adenine phosphoribosyltransferase.
The mechanism of reaction of adenine phosphoribosyltransferase from Ehrlich ascites tumor cells was investigated kinetically by initial velocity and product inhibition studies. The data are consistent with a mechanism in which 5-phosphoribosylpyrophosphate reacts with the enzyme to form an intermediate, presumably an enzyme-ribose 5-phosphate complex, with which adenine then reacts to form aden...
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The mechanism of reaction of human erythrocyte hypoxanthine-guanine phosphoribosyltransferase was investigated by initial velocity, product inhibition, and isotope exchange studies. Although initial velocity data are compatible with a mechanism involving binary enzyme-substrate complexes, the product inhibition and isotope exchange studies indicate that the reaction is ordered with the formatio...
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Key-words Disease name and synonyms Definition Excluded diseases Diagnosis criteria Differential diagnosis Prevalence Molecular defect Clinical description Management including treatment Etiology Diagnostic methods Antenatal diagnosis Unresolved questions References Abstract Adenine phosphoribosyltransferase (APRT) catalyzes the synthesis of AMP (adenosine monophosphate) from adenine and 5'-pho...
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Human adenine phosphoribosyltransferase has been purified 33,000-fold from erythrocytes to a specific activity of 9.58 pmoles of AMP formed per mg of protein per min. The native enzyme has a molecular weight of 34,000 and is composed of three subunits of equal molecular weight which appear to be associated by noncovalent forces. The highly purified enzyme is maximally active over a broad pH ran...
متن کاملAdenine Phosphoribosyltransferase from Monkey Liver
The structural requirements for the binding of purines to adenine phosphoribosyltransferase from monkey liver were explored by the determination of the K; values for 67 purines and purine analogues. Adenine was bound 40 times better than the most effectively bound analogues. Substitutions on the 6-amino group of adenine decreased binding to varying degrees. The hydroxylaminoand hydrazinopurines...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1966
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)96478-1